Sample Collection Quick-Reference Card¶
Feather Collection (DNA Sexing)¶
┌─────────────────────────────────────────────┐
│ FEATHER COLLECTION │
├─────────────────────────────────────────────┤
│ ☑ Use freshly plucked feathers ONLY │
│ ☑ 3–5 feathers per bird │
│ ☑ Small birds: 5–7 feathers │
│ ☑ Pluck from chest or flank │
│ ☑ Check: follicle bulb visible at base │
│ ☑ Store in paper envelope (NOT plastic) │
│ ☑ Label each envelope with bird ID │
│ ☑ Room temperature storage │
│ ☑ Ship within 4 weeks of collection │
└─────────────────────────────────────────────┘
Swab Collection (Disease Testing)¶
┌─────────────────────────────────────────────┐
│ SWAB COLLECTION │
├─────────────────────────────────────────────┤
│ ORAL SWAB: │
│ ☑ Insert into oropharynx + choanal slit │
│ ☑ Rotate 5–10 seconds │
│ ☑ Place into stabilization solution │
│ │
│ CLOACAL SWAB: │
│ ☑ Insert 0.5–1 cm into cloaca │
│ ☑ Rotate 3–5 seconds │
│ ☑ Place into stabilization solution │
│ │
│ ☑ Seal tube tightly │
│ ☑ Label with bird ID + date │
│ ☑ Store at 2–8°C → ship with cold pack │
└─────────────────────────────────────────────┘
Blood Collection (FTA Card / Filter Paper)¶
┌─────────────────────────────────────────────┐
│ BLOOD COLLECTION │
├─────────────────────────────────────────────┤
│ FTA CARD: │
│ ☑ Apply 1–2 drops blood to card │
│ ☑ Allow to air-dry 30 min (no direct sun) │
│ ☑ Store in sealed bag with desiccant │
│ ☑ Room temperature storage (years) │
│ │
│ FILTER PAPER (DBS): │
│ ☑ Apply 3–4 drops blood to card │
│ ☑ Air-dry completely (> 1 h) │
│ ☑ Store individually in paper envelopes │
│ ☑ Room temperature or refrigerated │
└─────────────────────────────────────────────┘
Shipping Quick Reference¶
┌─────────────────────────────────────────────┐
│ SHIPPING CHECKLIST │
├─────────────────────────────────────────────┤
│ ☑ Contact SENO for current instructions │
│ ☑ Feathers: paper envelopes → padded mailer│
│ ☑ Swabs: sealed tubes → absorbent bag │
│ ☑ Include submission form │
│ ☑ Use DHL / FedEx / UPS │
│ ☑ Arrange weekday delivery (Mon–Thu) │
│ ☑ Share tracking number with SENO │
│ ☑ Declare: "Lab Testing Samples, No Value" │
└─────────────────────────────────────────────┘
Expected Timelines¶
┌─────────────────────────────────────────────┐
│ TURNAROUND ESTIMATE │
├─────────────────────────────────────────────┤
│ Shipping: 3–5 business days │
│ Customs: 2–3 business days │
│ Lab Testing: 1–2 business days │
│ DNA Fingerprinting: 3–5 business days │
│ Reporting: Within 1 hour of QC │
│ ─────────────────────────────────────────── │
│ Total (sexing): 7–10 business days │
│ Total (fingerprint): 10–14 business days │
└─────────────────────────────────────────────┘
Specimen Stability¶
| Sample Type | Room Temp (20–25°C) | Refrigerated (2–8°C) | Frozen (–20°C) |
|---|---|---|---|
| Feather (dry) | 4 weeks | 6 months | Indefinite |
| Blood (FTA card) | Indefinite | Indefinite | Indefinite |
| Blood (EDTA tube) | 24 h | 7 days | — |
| Swab (dry) | 24 h | 3 days | — |
| Swab (stabilization buffer) | 7 days | 4 weeks | 6 months |
| Tissue (in RNA later) | 1 week | 4 weeks | Indefinite |
Common Errors & Prevention¶
| Error | Consequence | Prevention |
|---|---|---|
| Using molted feathers | No DNA (no follicle) | Always pluck fresh feathers |
| Plastic bag for feathers | Condensation → mold → DNA degradation | Always use paper envelope |
| Missing bird ID on sample | Sample cannot be processed | Label each envelope before collection |
| Swab tube leakage | Biohazard risk + sample loss | Double-check cap seal; use secondary container |
| Storage in direct sunlight | UV damage → DNA degradation | Store in opaque container or dark location |
| Over-drying blood spot | DNA may fragment | Follow drying time: 30–60 min, not > 2 h |
Contact for Questions¶
For sample collection questions not covered on this card:
- Email: [email protected]
- Tel: +86 13323237275
- Full guides: Feather Collection Protocol | Blood Sample Protocol | Swab Collection | International Shipping
This quick-reference card summarizes sample collection best practices for SENO Testing Center. For detailed protocols and species-specific guidance, refer to the full Sample Collection & Shipping Guide.